Benchling for CRISPR Screening

This application note outlines the key challenges in CRISPR screening and how Benchling has helped leading life sciences companies overcome these hurdles.

Overview

CRISPR screening is a revolutionary methodology to identify genes of interest underlying a specific phenotype. CRISPR can be scaled up for screening applications because of the versatility of CRISPR/Cas9 in disrupting target DNA and the ease of gRNA/plasmid generation. However, large-scale use of this technology requires scientists to track the viability of edited cells, maximize editing efficiency, minimize off-target effects, optimize vector design, and improve transformation efficiency.

This application note provides:

  • An overview of challenges faced in CRISPR screening

  • How Benchling’s capabilities can help overcome these complexities and improve productivity

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