CRISPR screening is a revolutionary methodology to identify genes of interest underlying a specific phenotype. CRISPR can be scaled up for screening applications because of the versatility of CRISPR/Cas9 in disrupting target DNA and the ease of gRNA/plasmid generation. However, large-scale use of this technology requires scientists to track the viability of edited cells, maximize editing efficiency, minimize off-target effects, optimize vector design, and improve transformation efficiency.
This application note provides:
- An overview of challenges faced in CRISPR screening
- How Benchling’s capabilities can help overcome these complexities and improve productivity